Electrochemical Fabrication of Poly (niacin) Modified Graphite Paste Electrode and its Application for the Detection of Riboflavin

J. G. Manjunatha1, *, C. Raril1, N. Hareesha1, M.M. Charithra1, P.A. Pushpanjali1, Girish Tigari1, D.K Ravishankar2, S. C. Mallappaji3, Jayarame Gowda4
1 Department of Chemistry, FMKMC College, Madikeri, Mangalore University Constituent College, Karnataka, India
2 Department of Chemistry, Sri Mahadeshwara Govt. First Grade College (University of Mysore), Chamarajanagar, India
3 Department of Chemistry, Government First Grade College for Women, (Affiliated to University of Mysore), Mysuru, India
4 Department of Chemistry, Bharathi College, Bharathi nagar, Mandya, India

© 2020 Manjunatha et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: ( This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Department of Chemistry FMKMC College, Madikeri, Mangalore University Constituent College, Karnataka, India Tel: +91- 08272228334; E-mail:



A poly niacin (NN) modified graphite paste electrode was prepared through a simple electrochemical polymerization route for the determination of Riboflavin (RF)


Electropolymerization technique was used for the preparation of a modified electrode. The electrochemical impedance spectroscopy (EIS) method was used to study the surface behaviors and conductivity of the bare and modified electrodes. Cyclic voltammetry (CV) and linear sweep voltammetry (LSV) were used to study the electrochemical behavior of RF using 0.2 M PBS of 7.5 pH.


Poly niacin modified graphite paste electrode (PNNMGPE) provides more affinity towards RF detection with high sensitivity and selectivity over bare graphite paste electrode. RF calibration curve was obtained by LSV between 5.0 µM to 65.0 µM with 0.782 µM limit of detection (LOD) and 2.7608 µM limit of quantification (LOQ), respectively. Selective analysis of RF in the presence of ascorbic acid (AA) and dopamine (DA) was performed at PNNMGPE by the LSV method.


The developed PNNMGPE was tested for RF analysis in a multivitamin tablet with good results. Furthermore, the developed electrode shows good stability and reproducibility.

Keywords: Poly niacin, Graphite paste electrode, Riboflavin, Voltammetry, Electropolymerization technique, Electrochemical impedance spectroscopy (EIS).