Electrochemical Fabrication of Poly (niacin) Modified Graphite Paste Electrode and its Application for the Detection of Riboflavin
J. G. Manjunatha1, *, C. Raril1, N. Hareesha1, M.M. Charithra1, P.A. Pushpanjali1, Girish Tigari1, D.K Ravishankar2, S. C. Mallappaji3, Jayarame Gowda4
Identifiers and Pagination:Year: 2020
First Page: 90
Last Page: 98
Publisher Id: TOCENGJ-14-90
Article History:Received Date: 28/08/2020
Revision Received Date: 11/12/2020
Acceptance Date: 14/12/2020
Electronic publication date: 31/12/2020
Collection year: 2020
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A poly niacin (NN) modified graphite paste electrode was prepared through a simple electrochemical polymerization route for the determination of Riboflavin (RF)
Electropolymerization technique was used for the preparation of a modified electrode. The electrochemical impedance spectroscopy (EIS) method was used to study the surface behaviors and conductivity of the bare and modified electrodes. Cyclic voltammetry (CV) and linear sweep voltammetry (LSV) were used to study the electrochemical behavior of RF using 0.2 M PBS of 7.5 pH.
Poly niacin modified graphite paste electrode (PNNMGPE) provides more affinity towards RF detection with high sensitivity and selectivity over bare graphite paste electrode. RF calibration curve was obtained by LSV between 5.0 µM to 65.0 µM with 0.782 µM limit of detection (LOD) and 2.7608 µM limit of quantification (LOQ), respectively. Selective analysis of RF in the presence of ascorbic acid (AA) and dopamine (DA) was performed at PNNMGPE by the LSV method.
The developed PNNMGPE was tested for RF analysis in a multivitamin tablet with good results. Furthermore, the developed electrode shows good stability and reproducibility.